Polymerases and proteinases makeup the components of viral proteins like nucleocapsid spike Releases positive sense single stranded RNA in to type-2 pneumocyte. Spike surface glycoprotein and after binding the host cell is engulfed in to the cell and Type-2 pneumocyte cells that could bind to human cells (Hoffmann et al., 2020 VerdecchiaĮt al., 2020). (S-Spike), it binds specifically to angiotensin-converting enzyme 2 (ACE2) to access host The SARS-CoV-2 has a surface spike like glycoprotein Viral replication and expression in host cells (Khan et al., 2020). The non-structural protein 16 (nsp16) or 2′-OMTase is the crucial protein responsible for (M) protein, Spike (S) protein and Envelop (E) protein and several non-structural proteins Nucleotides and it encodes with four structural proteins, Nucleocapsid (N) protein, Membrane The coronavirus genome is comprised of ∼30000 Coronaviruses are enveloped, single stranded RNA viruses whichīears club shaped glycoproteins on their surface (Prajapat et al., 2020 Sarma et al., 2020).Ĭorona virus is contagious and spreads through inhalation, ingestion of viral droplets SARS-CoV-2 caused diseases are characterized by a lower respiratory ailment like bronchitis,īronchiolitis, and pneumonia (Bogoch et al., 2020 Elfiky & Azzam, 2020). In lungs which leading to fatal respiratory distress (Elfiky, 2020 Galante et al., 2016 Joshi et al., 2020 Tse et al., 2004 Yi et al., 2020). Theįindings of the study confirmed that the final vaccine construct of chimeric peptide couldĪble to enhance the immune response against nCoV-19.ĬOVID-19 pandemic is result of the infection caused by Severe Acute Respiratory SyndromeĬoronavirus 2 (SARS-CoV-2) and it attacks the vital organs of body and targets pneumocytes The immune simulation results showed significant response for immune cells. The MD simulations confirmed stability of the binding Molecularĭocking study revealed the protein-protein binding interactions between the vaccineĬonstruct and TLR-3 immune receptor. Results subjected to the modeling and docking studies of vaccine were validated. Protein adjuvant and the construct was computationally validated in terms of antigenicity,Īllergenicity and stability on considering all critical parameters into consideration.
Final vaccine construct was designed in silico composed of 425 amino acids including the 50S ribosomal Most important immunogen of all the proteins and IEDB server gives the prediction andĪnalysis of B and T cell epitopes. Spike glycoprotein, of novel corona virus (SARS-CoV-2) strains. NCBI was used for the retrieval of surface The vaccineĬandidate was designed using B and T-cell epitopes that can act as an immunogen andĮlicits immune response in the host system. Is designed using in silico tools that potentially triggerīoth CD4 and CD8 T-cell immune responses against the novel Coronavirus. In the present study, a novel multi-epitope vaccine
The best therapeutic strategy to find an effective vaccine against SARS-CoV-2 is toĮxplore the target structural protein.